FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16453206
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTAT68600.018.8915188
CACTATA73100.017.6300759
AACACTA75250.017.3177137
ACCAAAC95850.014.2723183
TCGTGTG72950.013.6203033
AAACACT108500.013.2051056
TACCAAA128750.011.5759352
CCAAACA118200.011.4513734
ATACCAA150750.010.6784081
CAAACAC129000.010.6042775
TTCGTGT101050.010.3315312
GTTCGTG138300.08.5601361
GTGTGCT112850.08.1663055
CGTGTGC129100.07.58455044
CTATAGC45750.06.64112210-14
GATCGGA142100.06.60401771
TGTGCTT154400.06.5282876
AGTACTA48700.06.061656530-34
CTCTAGG20252.0978956E-56.0601431
GTATCGA110750.05.80103161
Produced by FastQC (version 0.11.5)