FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14729100
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA111950.023.4730477
TGGCTCA115000.022.850276
ATGGCTC117500.022.0581845
CTCAAAC131600.020.296259
TCTGATG153950.016.9833581
GATGGCT158750.016.5083854
GCTCAAA172300.015.6688228
CCACTCA49450.014.7045889
CTGATGG235300.011.4139972
TGATGGC254750.010.9374273
GCCACTC69150.010.7234738
CTCGGGT55450.09.8164820-24
AGCCACT81300.09.1211047
CTAGCTC17450.08.99403245-49
CCTCTAG21400.08.88152750-54
CTCTAGG16600.07.89333950-54
AAGCCAC102200.07.8193096
AGGTCCT83000.06.86974245-49
CCGTTAA66150.06.85689744
GGTCCTC89300.06.691446345-49
Produced by FastQC (version 0.11.5)