FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16758006
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT144050.050.5260854
AGTGGTA148750.049.026435
AGCAGTG173100.042.5512242
GTGGTAT178850.040.8156556
GCAGTGG204150.035.9367523
GTATCAA213900.034.935299
GGTATCA212900.034.930318
AAGCAGT223000.033.9321631
TGGTATC219100.033.8104367
CTTAGTC47300.028.918138
TTAGTCC51950.026.7454269
CCTTAGT55600.025.2486177
TCCTTAG89950.015.7667736
GCTCTCT129150.014.5499063
CTACCTC119350.012.3991721
TGCTCTC166550.011.5425072
CTCCTTA137400.011.4221425
TACCTCC134800.011.163232
CTCTCTC139000.010.9799124
CAACGCA153100.010.34485110-14
Produced by FastQC (version 0.11.5)