FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19705556
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT113100.035.5216874
AGTGGTA139350.029.1403065
AGCAGTG149800.027.4471342
GCAGTGG164550.024.9405233
GTGGTAT174850.023.5122156
TGGTATC218100.018.9486947
GGTATCA219450.018.8960698
AAGCAGT227350.018.4454121
GTATCAA236800.017.663599
CTTAGTC44450.07.77416948
TTAGTCC44850.07.54431729
CAACGCA143050.06.623070710-14
GCAGAGT141650.06.322617515-19
TCTTGGG145750.05.986668635-39
AACGCAG165750.05.663897510-14
CTCTCTA81650.05.20256854
ACGTTAA47401.1823431E-105.1644324
AACACTA73550.05.09030447
Produced by FastQC (version 0.11.5)