FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18395926
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT84050.038.4624824
AGTGGTA108300.030.3820085
AGCAGTG122350.026.6022662
GTGGTAT133850.024.4211546
GGTATCA141500.023.251178
TGGTATC150100.022.400877
GCAGTGG151650.021.4604513
GTATCAA156150.021.254169
AAGCAGT174250.019.5001351
GCAGAGT109750.06.664688615-19
TCTTGGG102850.06.60789835-39
CAACGCA113400.06.589820410-14
TCTAATG72700.06.43735744
AGTGAGT30003.3863034E-75.54267171
GTGAGTA35506.704067E-85.2738672
AACGCAG155450.05.020278510-14
Produced by FastQC (version 0.11.5)