FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14886505
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT95300.038.978134
AGTGGTA96350.037.955635
AGCAGTG127200.028.9955652
GTGGTAT137500.026.8583226
GGTATCA137700.026.7670338
GTATCAA144050.025.4371649
GCAGTGG146300.025.2919923
TGGTATC146400.025.1271977
AAGCAGT159550.023.278351
TCTTGGG102900.07.29032535-39
GCAGAGT111950.07.07352215-19
CAACGCA134750.06.20785510-14
TATCAAC146900.05.83162210-14
AACGCAG142600.05.82573210-14
Produced by FastQC (version 0.11.5)