FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14886505
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAAC121550.019.9605629
GGCTCAA128100.018.8279787
TGGCTCA132200.018.1891676
ATGGCTC128250.018.0196135
TCTGATG128800.017.5715431
GCTCAAA170350.014.4539868
GATGGCT166950.014.1447084
CCTCTAG12100.010.59081850-54
CTGATGG244150.09.6173552
TAGTGTC26600.09.4729275
TGATGGC277050.08.8361353
CTCGGGT59200.08.02619220-24
GTAGTGT36750.07.44445324
CTCTAGG11400.06.94676750-54
AGTAGTG42500.06.77661233
CTAGGAT13050.06.62011650-54
AACACAG81150.06.33447210-14
CTAGCAC10100.05.987600350-54
AGGTCCT92550.05.616165645-49
GGGTGGG92100.05.612667625-29
Produced by FastQC (version 0.11.5)