FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14152768
Sequences flagged as poor quality0
Sequence length150
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA66500.048.6093145
CAGTGGT79800.040.8683434
GTATCAA92750.036.016039
GTGGTAT100750.032.727456
GGTATCA109250.030.444748
TGGTATC115150.028.7597857
AGCAGTG126600.025.8757212
AAGCAGT135200.023.6972561
GCAGTGG146550.022.3532333
TCTTGGG87100.07.68764635-39
TATCAAC101450.07.224131610-14
GGGGGGG57600.06.99934344
GCAGAGT106200.06.94169715-19
CAACGCA111400.06.733989710-14
AGGGGGG47850.06.4699551
GTATAGA46851.8189894E-125.5323251
TATAGAC43656.366463E-115.44307662
GAGCACA156350.05.20322139
AACGCAG146900.05.1850610-14
TCGGAAG174050.05.1293513
Produced by FastQC (version 0.11.5)