FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14152768
Sequences flagged as poor quality0
Sequence length150
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCTC103850.025.5134835
GGCTCAA108450.024.2985277
TGGCTCA108500.024.287336
CTCAAAC113550.023.7144419
TCTGATG111450.023.3458671
GATGGCT133450.019.800144
GCTCAAA167250.016.2724938
CCTCTAG11350.013.32130150-54
GATCTAG6655.6323497E-611.9094943
CTCTAGG8700.011.58595950-54
TCTAGCC7501.7043803E-611.5198935
ATCTAGC7551.8466599E-611.4433994
CTGATGG243450.011.4184122
CTAGGAT8700.011.25493250-54
TCTAGGA8850.010.90146350-54
TGATGGC279350.010.3094053
CTAGCCG8356.9650356E-59.48494156
CTCGGGT56300.09.36102620-24
AACACAG71400.08.22843610-14
GGTCTAA11650.00246377526.8053371
Produced by FastQC (version 0.11.5)