FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17827932
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC358480.20107772455044143No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC232070.1301721366224641No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA175950.057.289217
TGGCTCA183750.054.3862276
CTCAAAC189000.053.4856159
ATGGCTC190950.051.9207655
GCTCAAA220300.045.363518
TCTGATG224750.044.1598741
GATGGCT250800.039.501324
CTGATGG313450.032.050522
TGATGGC342600.029.3161933
CTCGGGT115150.017.13206920-24
CCTCTAG30950.016.93524450-54
CTCTAGG23150.015.239326550-54
GGTCCTC147250.013.29020545-49
AGGTCCT153800.013.02381945-49
GGGTGGG154900.012.88513125-29
AACACAG165500.012.70333610-14
TCGGGTG157700.012.57416825-29
AGCGACC163800.012.52807515-19
ACACAGC170250.012.38274210-14
GGTGGGA164000.012.23162825-29
Produced by FastQC (version 0.11.5)