FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21002057
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA131000.039.5711445
CAGTGGT132350.039.058714
GTGGTAT179500.029.039666
AGCAGTG185400.028.312192
GTATCAA201950.026.2727729
TGGTATC201200.025.7645157
GGTATCA210750.024.9366178
GCAGTGG213900.024.6728253
AAGCAGT235250.022.6195561
TCTTGGG141150.07.732321735-39
GCAGAGT154600.07.366988715-19
CAACGCA183300.06.543390810-14
AACGCAG214550.05.704409610-14
TATCAAC222300.05.39542710-14
Produced by FastQC (version 0.11.5)