FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15366547
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCTC97600.016.2820665
TGGCTCA98800.016.1572976
GGCTCAA117050.013.8224127
GATGGCT134700.011.4238694
CTCAAAC144750.010.9785149
TCTGATG155350.09.3986151
GCTCAAA209350.08.0716958
TGATGGC218500.07.6349043
CTGATGG217500.07.0421122
CTCGGGT44900.06.3418220-24
Produced by FastQC (version 0.11.5)