FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13684775
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT63200.030.5289274
AGTGGTA63550.030.36095
GTGGTAT94250.020.776786
AGCAGTG94800.020.3541052
GTATCAA103950.018.5610529
TGGTATC110450.017.7945737
GGTATCA115400.016.9065178
GCAGTGG118050.016.6503073
AAGCAGT127300.015.1010941
CCACTCA29850.09.6473479
TACACGT32555.456968E-126.6358391
GCCACTC52200.05.9304638
CCCTTAA41152.3483153E-95.24862154
AGCCACT61600.05.14236167
TACTTGG61800.05.12613251
Produced by FastQC (version 0.11.5)