FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16867684
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACATTA67500.013.3262369
CTAGCTA14700.012.23848725-29
ATCTAGC17950.010.10278725-29
TCTAGCT19650.09.08226725-29
TATCTAG20850.08.8356625-29
TACAATC121500.08.4702652
CAATCCA110850.08.4391984
CCACATT122750.07.9142018
ATACAAT133450.07.61835431
ACAATCC147650.07.35939553
AATCCAC134950.07.252335
GGCCCTA26950.06.78219945-49
ATCCACA151450.06.50943856
CCCCTAA31300.06.1273394110-114
TCCACAT180900.05.84754477
CTATCTA38450.05.38964320-24
CCCTAAT38650.05.1859694110-114
Produced by FastQC (version 0.11.5)