FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12986226
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT73850.046.500884
AGTGGTA79150.043.478415
GTGGTAT101500.034.188056
AGCAGTG106200.032.9480862
GGTATCA111850.031.4750358
TGGTATC111650.031.337977
GTATCAA117850.029.4449489
GCAGTGG126550.027.4222663
AAGCAGT137600.025.7956751
GCAGAGT97300.07.709837415-19
GGGGGGG30700.07.27012352
GGTACAA61650.07.2406411
TCTTGGG101600.07.22816835-39
TATCAAC114250.07.03233110-14
CAACGCA111250.06.9372310-14
GGTACGA75200.06.89339351
GGTTCTA41100.06.65671871
GATCCTA14555.445932E-46.4327811
GGTTCGA95450.06.1852371
AACGCAG130850.06.07416710-14
Produced by FastQC (version 0.11.5)