FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15971038
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA134100.018.8443857
ATGGCTC143250.017.1886025
CTCAAAC150300.017.1485089
TGGCTCA156700.016.356136
GCTCAAA180650.014.7057758
TCTGATG179000.014.0433651
GATGGCT207550.012.41874
CCTCTAG17050.010.30309650-54
CTGATGG257350.010.1005852
TGATGGC320750.08.5073153
CTCTAGG14750.08.39534950-54
CTCGGGT71950.07.46519720-24
TCTAGGA17900.06.354864650-54
AGGTCCT96350.05.94782945-49
AACACAG101950.05.748300610-14
CTAGGAT19650.05.71563350-54
GGTCCTC97050.05.60819845-49
GGGTGGG103800.05.354483625-29
ACACAGC112200.05.312999210-14
CAGGTCC109150.05.289903645-49
Produced by FastQC (version 0.11.5)