FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16315988
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT93450.032.744684
AGCAGTG126950.024.842952
AGTGGTA125550.024.430035
GCAGTGG133250.023.2346633
GTGGTAT159450.019.6876136
TGGTATC166800.019.2085747
GGTATCA174250.018.4267168
AAGCAGT181850.017.4902611
GTATCAA204150.015.55159959
GTCCCTA28100.012.5552373
GTGAGTA39500.08.7499562
AGTGAGT38200.08.7046511
TAACCCC45100.07.8217779
TTAGCAC47600.07.71426157
TAGCACA55500.06.8749378
TCTTGGG105500.06.20988235-39
CCCTATC69650.06.09904775
GCAGAGT116500.06.006727715-19
CCTATCG68350.05.793696
CAACGCA122950.05.773551510-14
Produced by FastQC (version 0.11.5)