FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16315988
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCTCA114550.018.4165526
GGCTCAA120750.017.350627
ATGGCTC123650.016.7118135
CTCAAAC139350.015.5506099
GATGGCT163300.012.8353874
GCTCAAA175050.011.927498
TCTGATG176600.011.8442491
CTGATGG247850.08.9840642
AGTGAGT38000.08.7918441
TGATGGC257750.08.6076293
GTCCCTA26400.08.1855683
TAACCCC43450.08.1188449
GTGAGTA44050.07.68874172
CTCGGGT60850.07.145906420-24
TTAGCAC50250.06.7339677
TAGCACA58800.05.87728938
GTGTACC83200.05.67408371
GGGTGGG89100.05.60750825-29
CCTCTAG23800.05.565702450-54
ATACGAG84450.05.45623978
Produced by FastQC (version 0.11.5)