FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18614964
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG88200.038.214257
GTAATAC125450.026.5802126
GATCTGT127300.026.2387281
TCTGTAA132950.025.334523
ATCTGTA137000.024.8482462
CTGTAAT140550.023.9133914
ATACGAC145550.023.4056959
TGTAATA170200.020.0435715
GACTAGC11250.018.5524755
AATACGA204100.016.8668128
ACTAGCC13300.015.7034266
GGACTAG14700.014.6878794
CTAGCCT14550.014.3543377
CTCACTA52100.013.06776615-19
GACTCAC54450.012.50974510-14
CATCTAG31450.012.36809145-49
CGACTCA56400.012.12829210-14
CAGTGGT70450.011.646064
ACTCACT59050.011.48645810-14
ACTATAG64150.010.70285515-19
Produced by FastQC (version 0.11.5)