FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14163594
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGAGAATCTCGTAT178380.1259426103289885TruSeq Adapter, Index 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA195000.085.65325
CAGTGGT214550.077.88144
GTGGTAT225250.074.2137766
AGCAGTG230750.072.4809342
GGTATCA247450.067.7595758
TGGTATC249400.067.114317
GTATCAA252400.066.573319
AAGCAGT260650.063.945431
GCAGTGG318900.052.581293
TCTTGGG197150.016.52189635-39
CAACGCA215400.016.20998410-14
GCAGAGT218250.015.56283515-19
GAGCACA202850.014.8350819
GATCGGA204500.014.716321
AACGCAG236350.014.65129410-14
CCACTCA50150.014.4990359
AGAGCAC206350.014.4439038
TATCAAC245900.014.27551210-14
TCAACGC257550.013.65213610-14
ATCAACG262700.013.41190410-14
Produced by FastQC (version 0.11.5)