FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14163594
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC543550.3837655894400814No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC347970.24567916871946488No Hit
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG143450.1012807907371533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCTC196750.077.616985
GGCTCAA201600.075.9290857
TCTGATG222150.069.169081
CTCAAAC227150.067.8320549
GCTCAAA237450.064.616768
GATGGCT237100.064.376974
TGGCTCA284800.054.454856
CTGATGG318100.048.5387462
TGATGGC339700.045.6961333
CTCGGGT138800.021.47493720-24
TCGGGTG165300.018.19004625-29
AGGTCCT169050.017.76025845-49
GGTCCTC170500.017.7021245-49
CCTCTAG46650.017.4708150-54
AACACAG188950.016.93385510-14
TGGGAAC182200.016.62161430-34
AGCGACC185300.016.56851815-19
CCTCGGG186400.016.32317420-24
CTCTAGG31450.016.29962550-54
AACACGT110200.016.28241330-34
Produced by FastQC (version 0.11.5)