FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21795405
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC1161510.5329150800363655No Hit
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG724800.33254715844922356No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA478860.21970686023040178No Hit
AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA324990.1491094109056473No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA263700.07.5488211
AGAGCAC310000.06.68812758
GAGCACA319250.06.5394459
TCGGAAG317150.06.51484253
GAGAGAC106200.06.2368882
GAGACGA112600.05.8820164
ATCGGAA370200.05.77596662
CGGAAGA436250.05.000164
Produced by FastQC (version 0.11.5)