FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17096577
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA134100.021.2064297
ATGGCTC134700.021.165485
CTCAAAC140750.019.8974729
TGGCTCA149200.019.0116716
TCTGATG162050.016.9904691
GATGGCT181600.015.8581424
GCTCAAA204150.014.0355018
CTGATGG277250.010.466162
TGATGGC305650.09.9638673
CCTCTAG17250.08.59754350-54
CTCGGGT66750.08.41371120-24
CTCTAGG14350.07.124143650-54
AACACAG99200.06.168792710-14
GGTCCTC104050.05.978115645-49
AGGTCCT105150.05.901883645-49
TCTAGGA18350.05.88506750-54
CTAGGAT18050.05.50424950-54
GGGTGGG112850.05.473759725-29
ACACAGC120250.05.376308410-14
CGACTTA33951.4303077E-75.3014316
Produced by FastQC (version 0.11.5)