FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17586879
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA99100.034.0012745
CAGTGGT107800.031.190414
GTGGTAT149500.022.586096
AGCAGTG151100.022.401072
GCAGTGG163950.020.7746873
GTATCAA167050.020.1270379
TGGTATC173050.019.4707957
GGTATCA177950.019.0560268
AAGCAGT191150.018.3769651
GAGCCCT68150.07.0781276
CCCTTAC70700.06.72100079
TGAGCCC72500.06.3557235
GCCCTTA76750.06.2850088
TCTTGGG111100.06.07852535-39
CAACGCA143200.05.389657510-14
GCAGAGT148550.05.24401615-19
Produced by FastQC (version 0.11.5)