FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17586879
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA132950.019.4415077
CTCAAAC142800.018.806569
TGGCTCA142700.018.718626
ATGGCTC146450.017.6493595
TCTGATG164050.015.6368651
GATGGCT186650.013.9638274
GCTCAAA204600.013.68888
CCTCTAG17900.010.77965550-54
CTCTAGG16200.010.22200150-54
CTGATGG277250.09.7905812
TCTAGGA17900.09.65342250-54
CTAGGAT19350.09.004456550-54
TGATGGC312750.08.9323993
CTCGGGT67400.08.05461820-24
AACACAG109150.05.7916510-14
CCCTTAC71350.05.6509689
AGGTCCT109950.05.38274345-49
GGGTGGG121600.05.198660425-29
TCGGGTG111800.05.19067425-29
TGAGCCC76450.05.1797535
Produced by FastQC (version 0.11.5)