FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15572684
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC165610.10634647180922698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA139100.015.3192579
GATCGGA143150.015.0116631
AGAGCAC157150.013.4686988
TCGGAAG173150.012.368713
ATCGGAA199200.011.1856082
AAGAGCA232150.09.7376697
GAAGAGC256650.09.0323696
GGAAGAG280150.08.2490035
CGGAAGA284250.07.8891864
AGTCACA69200.06.304631725-29
CTCGTAT78150.05.713923540-44
CACACGT87500.05.101003610-14
CAGTCAC90350.05.083767425-29
TCTCGTA93000.05.06484940-44
Produced by FastQC (version 0.11.5)