FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11941874
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA77200.037.2125855
CAGTGGT84300.034.0784264
GTGGTAT105350.027.747626
AGCAGTG106850.027.4961952
GTATCAA116600.024.9445239
GCAGTGG123250.023.60143
GGTATCA125100.023.364758
TGGTATC130400.022.4172657
AAGCAGT138650.021.487731
CTAGGTA7650.06.9650946120-124
CAACGCA100550.06.57290510-14
TCTTGGG98700.06.302232735-39
GCAGAGT103000.06.1789315-19
CCTAGGT8300.06.072629120-124
AACGCAG109750.05.9956810-14
TATCAAC126750.05.532325710-14
GCCTAGG10002.0008883E-115.1842895120-124
Produced by FastQC (version 0.11.5)