FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13370676
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTAT217310.16252730976354524TruSeq Adapter, Index 5 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA184950.022.072639
AGAGCAC193500.021.320588
GATCGGA231350.017.5981621
TCGGAAG257250.016.2336373
AAGAGCA283000.014.9594727
GAAGAGC284350.014.8873916
ATCGGAA288600.014.5701712
GGAAGAG334000.012.8037675
CGGAAGA350100.012.1956744
GTGAGTA47450.09.25639252
TAACCCC48500.09.0555219
AGTGAGT51200.09.0554611
GAGTCAA101550.07.72784335-39
AGAGTCA104650.07.333624430-34
CAGAGTC106850.07.290434430-34
CTCGTAT105450.07.12799840-44
AGTCACA118200.06.882806325-29
TCTCGTA117500.06.764642740-44
AATCTCG119350.06.70804740-44
AGGGGGG62750.06.5805421
Produced by FastQC (version 0.11.5)