FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13370676
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTCTTCAGTGTAGATCT239060.17879425094138846Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA229900.017.5212631
AGAGCGT228400.017.4309488
GAGCGTC228450.017.2065359
AAGAGCG251550.016.2274657
TCGGAAG265100.015.4240963
GAAGAGC289350.014.1323236
ATCGGAA294150.014.0245552
GGAAGAG343350.012.01460555
CGGAAGA351150.011.7680564
GTGAGTA44100.09.9585152
TAACCCC51750.08.207939
AGTGAGT51800.08.0974341
TAGATCT110300.07.59685840-44
AGTGTAG111650.07.376049540-44
CAGTGTA121300.06.848714435-39
GTAGATC129550.06.70141440-44
AGATCTC130000.06.64482345-49
TCAGTGT134750.06.336069635-39
GAGTGTT132850.06.29679625-29
GTGTAGA141300.06.19509540-44
Produced by FastQC (version 0.11.5)