FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15983063
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA89300.027.329065
CAGTGGT94100.026.3175354
GTGGTAT126300.019.7219186
AGCAGTG132600.019.2760012
GTATCAA149250.016.8340079
GCAGTGG158200.016.0193613
GGTATCA161050.015.7793968
AAGCAGT168900.015.5346851
TGGTATC160600.015.4201767
AACACTA39550.012.3776687
CACTATA44750.011.42198859
ACACTAT49000.010.7251458
AAACACT92000.05.9470586
Produced by FastQC (version 0.11.5)