FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13593834
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTAGCCATCTCGTAT157450.11582457164034812TruSeq Adapter, Index 15 (97% over 37bp)
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG156600.11519928814784704No Hit
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC156480.11511101283125863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT139300.068.164764
AGTGGTA156850.060.4448745
AGCAGTG177750.053.6358872
GTGGTAT189650.050.180716
GCAGTGG190650.050.0701983
TGGTATC211150.045.384167
TAGAGTC39500.043.7393535
GGTATCA220350.043.6853378
GTATCAA223000.043.101639
AAGCAGT224700.042.143741
TAAGTAG45850.037.2649961
GTAGAGT58300.029.6352714
GAGTCTT67850.025.6792817
AGAGTCT70300.024.7809686
AGTAGAG74450.023.8838773
AGTCTTG95750.018.121568
GCTAGTC19450.017.25168885-89
GCTAGCT19250.016.681946140-144
CGCTAGC19000.016.522488140-144
AAGTAGA109550.016.1940842
Produced by FastQC (version 0.11.5)