FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13593834
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC261590.1924328338863046No Hit
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG229240.16863527978935156No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC183760.1351789348023523No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAGACGGAGTGTAGATCT168190.12372521247500888Illumina Single End PCR Primer 1 (96% over 32bp)
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA156560.11516986304231758No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA138400.058.1917347
ATGGCTC146800.053.930685
TGGCTCA152250.052.331346
CTCAAAC160600.050.3259779
GCTCAAA184300.044.128258
GATGGCT183400.043.1680644
TCTGATG191550.040.5689431
CTGATGG237200.033.377982
TGATGGC256100.031.3068893
TCTACTA77300.021.489182
CCTCTAG25550.019.4205850-54
ACTATTC87450.019.076685
CTCGGGT81050.018.91506420-24
CTACTAT90400.018.6927873
GACCTAG18250.018.6796285-89
ACCTAGC19400.017.6464785-89
CTATTCT98550.017.3659976
CTAGCGA19750.017.18449490-94
GCTAGCG20150.016.62925190-94
CTCTAGG18900.016.43712850-54
Produced by FastQC (version 0.11.5)