FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16068172
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACAC38550.020.949083
TTGACTC60300.015.7597868
TGACTCC62650.015.2828379
TCTCACA57050.014.0293852
TCACACA58500.013.558014
CACACAT69400.011.8259965
ATCTCAC78400.010.3366481
TGGGGGG42050.09.6360671
GGGGGGG70150.09.3537343
CATTCAC87150.09.3343589
CAACGGA115900.08.8650811
TTTGACT117050.08.8569897
ATTTGAC124050.08.5311486
ACGGAAC124950.08.1945173
AGGGGGG46650.07.91036131
GGGGGGT28750.07.52410272
ACACATT115750.07.15259746
GTACGGG68300.06.99199341
TAATTTG164350.06.80019144
CACATTC118900.06.78158477
Produced by FastQC (version 0.11.5)