FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16209626
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCTCA112750.022.7962366
GGCTCAA117950.022.0969737
ATGGCTC120350.021.775435
CTCAAAC123050.021.6487549
TCTGATG138350.018.306131
GCTCAAA177850.014.7760548
GATGGCT177100.014.5539654
CCTCTAG11050.013.29132850-54
CTGATGG244450.010.3713262
CTCTAGG9500.09.85190650-54
TGATGGC297800.08.9700913
CTCGGGT72750.07.402185420-24
TCTAGGA12950.07.22726750-54
CTAGCAC9150.07.08144550-54
CTCTAGC9900.06.835860350-54
CTAGGAT13950.06.605964750-54
AACACAG88500.06.442922610-14
ACACAGC98100.06.07662410-14
GGGTGGG98050.05.88896625-29
GGTGGGA102250.05.816061525-29
Produced by FastQC (version 0.11.5)