FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14543528
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC157870.10855000244782423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA152600.030.8101547
CTCAAAC165750.028.7133059
ATGGCTC178550.025.9690935
GCTCAAA201350.023.3147748
TGGCTCA225150.021.2977166
GATGGCT238000.019.4516224
TCTGATG254550.018.3204251
CTGATGG268600.017.641512
TGATGGC332150.014.52323
CTCGGGT65200.014.09047120-24
GGGTGGG80850.011.47043925-29
GATCGGA124300.011.3075331
TCGGGTG87350.010.55094325-29
GGTCCTC92350.010.29124945-49
GGTGGGA93800.010.22458325-29
CCTCGGG97600.09.91452320-24
AGCGACC103950.09.392467515-19
TCGGAAG164500.09.0161913
CCTCTAG31150.09.01415650-54
CGGGTGG106400.08.98670925-29
Produced by FastQC (version 0.11.5)