FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13534010
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA106150.027.5371137
CTCAAAC108750.027.0099669
TGGCTCA113850.025.676226
ATGGCTC111550.025.5601675
TCTGATG128250.021.2297881
GCTCAAA154150.019.4296688
GATGGCT150750.019.0654834
CTGATGG214500.013.7749352
TGATGGC239700.012.2314043
CCTCTAG15450.011.46273350-54
CTCTAGG11100.010.50687650-54
CTCGGGT60650.010.06587220-24
TCTAGGA14500.08.4403950-54
CTAGGAT15550.08.05564750-54
AACACAG83750.07.89141410-14
ACACAGC90100.07.41515410-14
GGTCCTC90000.07.19925745-49
AGGTCCT88200.07.00335945-49
GTGCTAG11700.00260661436.76896768
GGGTGGG101550.06.621468525-29
Produced by FastQC (version 0.11.5)