FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13757450
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCTCA87400.019.1945866
GGCTCAA101000.016.9657887
ATGGCTC98600.016.5031095
CTCAAAC111450.015.5031969
TCTGATG124400.012.9055091
GATGGCT132900.012.6832474
GCTCAAA160050.011.0212398
CTGATGG191750.08.9078662
TGATGGC207050.08.7677893
CTCGGGT51250.06.686410420-24
CCTCTAG15700.06.419609550-54
GGTCCTC68100.05.66635745-49
CTCTAGG12650.05.577194750-54
AACACAG76550.05.304280810-14
AGGTCCT73650.05.180711345-49
Produced by FastQC (version 0.11.5)