FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12922055
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCTC93950.022.0699485
CTCAAAC95550.021.5492699
TGGCTCA97700.021.001776
GGCTCAA102800.020.0300437
TCTGATG104350.019.4505061
GATGGCT123950.016.9607584
GCTCAAA132100.015.914238
CCTCTAG9950.013.747498550-54
CTCTAGG7600.011.36739250-54
TCTAGGA8450.010.22392750-54
CTGATGG216600.010.0762
CTAGGAT8750.09.37972250-54
TGATGGC249700.08.7081853
CTAGCAC7100.08.3147450-54
CTCGGGT59350.07.49644820-24
AACACAG68500.06.999793510-14
CTCTAGC9400.06.280282550-54
CCTAACT13000.0078293426.09181269
TCTAGCA10950.05.91727250-54
AAACACA88850.05.704489710-14
Produced by FastQC (version 0.11.5)