FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12186716
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG1547221.2695955169546906No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA1147580.9416646781626814No Hit
AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA831150.6820131034480494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT53750.010.621721
TCCCTAC42550.010.4916499
TTCCCTA50050.010.0703748
GATCGGA194800.09.5343351
TCGGAAG200950.09.4950443
ATCGGAA212200.09.0603712
AGAGCGT221250.08.4939348
CACTCTT76350.07.92228272
AAGAGCG250900.07.43289237
GAGCGTC263750.07.1252429
CGGAAGA294650.06.744574
TCCTCCT353800.06.55682231
TCCTCTT331750.06.55462174
CTTCCTC368600.06.2122932
TCTTCCT369250.06.16504531
TTCCTCT301450.06.162313
GGAAGAG340650.06.15083935
ACTCTTT103650.06.04350763
GAAGAGC328000.05.9710196
CCTCCTC331200.05.78324272
Produced by FastQC (version 0.11.5)