FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17735719
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA91000.029.1126985
CAGTGGT97100.027.2096424
AGCAGTG133600.020.9640052
GTGGTAT130350.020.5450846
GCAGTGG146800.018.587453
TGGTATC153800.017.5997927
GTATCAA164050.016.8073259
GGTATCA166800.016.3575848
AAGCAGT192800.014.4381211
GTCCCTA23406.5944914E-76.2282851
GTCTACG84950.06.17622951
GTCTCCA103050.06.1521311
TCTTGGG105600.05.821970535-39
GTCTACA86300.05.572981
GTCTATA75950.05.5648641
GTCTATC104600.05.43397051
GTCTATT79650.05.3063581
GTCTTCG228750.05.22440771
GTCTTTG242500.05.10847761
GTCTGCG235050.05.0223741
Produced by FastQC (version 0.11.5)