FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14707221
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACTA76650.023.3876067
ACACTAT87350.021.0170338
CACTATA96450.018.8848029
CAAACAC106550.016.9597265
ACCAAAC126950.014.7452623
AAACACT124950.014.692736
CCAAACA129300.014.5887824
GCCCTTA65400.013.1004294
TAGCCCT66750.012.7274842
CTCAAAC100050.011.6571419
GGCTCAA109550.010.5806437
GTAGCCC84350.010.5125081
TGGCTCA108500.010.48397356
ATGGCTC108250.010.3751715
AGCCCTT94000.09.420833
GCTCAAA134750.08.9224028
TATTTAG119400.08.2605769
GATGGCT141750.08.126684
TCTGATG147250.07.81358671
CCCTTAT135250.07.5055135
Produced by FastQC (version 0.11.5)