FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10977067
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACTA28700.020.821667
ACACTAT32650.018.9643698
CACTATA32550.018.1381039
AAACACT54500.012.2859176
CAAACAC65500.09.5631085
ATCAAAC87100.07.8528733
TCAAACA92500.07.47227054
TTAGGCA28950.05.52108460-64
GCCCTTA38452.397428E-95.43031174
CTTGGGG63150.05.24502041
ACTATAG27600.05.060829610-14
Produced by FastQC (version 0.11.5)