FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15019938
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC305050.20309671051904474No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA138850.017.889819
AGAGCAC151600.016.2902378
GATCGGA181700.014.106771
TCGGAAG187400.013.5624343
ATCGGAA227950.011.9078742
AAGAGCA230250.011.7263847
CTAGTCC12000.011.03999990-94
CGCTAGG8200.010.88780470-74
GCTAGTC11500.09.89217490-94
GCTAGGG10000.09.07270-74
CTAGGGC9100.09.0197870-74
GGAAGAG288850.08.9984425
TCGCTAG13000.08.861539140-144
GAGCTAG11900.08.83361360-64
GAAGAGC306250.08.7928166
CGGAAGA312000.08.6769244
GAGCCCA67550.07.9940789
AGGCTAG16800.07.799999785-89
AGTCACA69600.07.59310425-29
GTTACCA59450.06.78217037
Produced by FastQC (version 0.11.5)