FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12200473
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAACC66600.010.3733258
CCAACCA75650.09.2274959
AGCCCAA85350.08.7690156
GGCCCTA18900.08.30073270-74
ATACAAG96550.08.2013291
GCCCAAC87150.08.1750197
TACAAGC106850.07.4106862
ACAAGCC125000.06.6225943
CTACTCA28500.05.74060545-49
AAGCCCA141350.05.70218665
TACGCTA30350.05.595956325-29
CTACCTA28950.05.451323545-49
CCTAAGC30350.05.216548130-134
Produced by FastQC (version 0.11.5)