FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11841465
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACTA38600.039.1628537
ACACTAT38750.038.4539578
CACTATA41250.036.1234139
AAACACT66950.022.9023786
ACCAAAC72250.020.727583
CAAACAC88550.017.153215
CCAAACA92200.016.7111344
TACCAAA97450.015.8123772
CCCCTTA30400.014.9207294
ATACCAA113550.014.39081
TATTTAG42950.010.22372159
TACCCCT44900.010.1032122
GATCGGA156400.010.0715461
ACTATAG32050.09.79188610-14
CTATAGC34250.09.58323610-14
TTAGGCA35250.09.27114260-64
AGAGCGT169500.09.2582558
AGTACTA35950.09.17061130-34
TCGGAAG179750.08.6918843
GTACTAA37100.08.61471330-34
Produced by FastQC (version 0.11.5)