FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16945327
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA89800.034.5561755
CAGTGGT95850.032.3750084
AGCAGTG144050.022.2975852
GTATCAA148400.021.0069689
GTGGTAT150950.020.9859626
GCAGTGG157500.019.888353
TGGTATC164600.019.2893757
GGTATCA164550.018.988968
AAGCAGT182350.017.560971
TCTTGGG110600.05.97581235-39
GCAGAGT126250.05.668450415-19
CAACGCA142600.05.129610-14
Produced by FastQC (version 0.11.5)