FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14450270
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA157350.014.5052421
GAGCACA150250.014.471889
AGAGCAC159400.013.7314938
TCGGAAG175650.013.1579863
ATCGGAA190350.012.255322
AAGAGCA212450.010.7432347
GGAAGAG262150.09.0360485
GCTAGTC9950.08.8281490-94
CGGAAGA282600.08.22934
CTAGTCC11100.08.17297490-94
GAAGAGC286000.08.0811196
GAGCTAG6950.08.08057660-64
AGTCACA55550.08.06192625-29
AGGCTAG11850.06.805063285-89
CAGTCAC69900.06.69527925-29
TCGCTAG11250.06.656140-144
CCAGTCA81550.06.056652525-29
CTCGTAT76200.06.009448540-44
CACACGT81650.05.573055710-14
GTCACAG91550.05.28498125-29
Produced by FastQC (version 0.11.5)