FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14450270
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA155650.014.1548351
TCGGAAG177850.012.3879673
ATCGGAA194400.011.8518512
AGAGCGT203900.010.73467358
AAGAGCG226350.09.7972167
GAGCGTC263650.08.4657699
GGAAGAG269700.08.2224695
CGGAAGA293050.07.9358474
AGTGTAG59100.07.870050425-29
GAAGAGC288650.07.80737926
GAGTGTA63850.07.216914725-29
TAGATCT71450.06.79188240-44
TAGCTAG7500.06.7290-94
AGAGTGT74400.06.561290325-29
TAGCTTC78250.06.238466330-34
TAGGGAA78450.06.22256215-19
GTAGCTT79500.06.10415130-34
CTAGCTA8201.0913936E-115.79512290-94
TCATTAA83600.05.649760765-69
GTAGGGA87100.05.406215-19
Produced by FastQC (version 0.11.5)