FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12718450
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC561510.4414924774638419No Hit
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG291450.22915528228675663No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGTAT185710.14601622053001742TruSeq Adapter, Index 5 (97% over 37bp)
CTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCT140970.11083897802012038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA195800.017.5769339
AGAGCAC207350.016.4936738
GATCGGA269600.013.558251
AAGAGCA283000.012.5426227
TCGGAAG291850.012.0396893
ATCGGAA326400.011.0963422
GAAGAGC321850.011.0279276
GGAAGAG359550.09.8916375
CGGAAGA391450.09.1234084
CTCGTAT90150.07.331759540-44
GTCACAC99000.07.010497625-29
TCTCGTA97250.06.974170740-44
AGTCACA98900.06.973908425-29
TCACACA104600.06.483933430-34
CAGTCAC108200.06.37448725-29
GGTACTA31154.7475623E-106.27390431
GGTTAAG32201.0477379E-96.06932071
CACACAG117050.06.064916630-34
GGTAACA69750.06.01888371
AATCTCG116250.05.933402540-44
Produced by FastQC (version 0.11.5)