FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005181671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005181671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12718450
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC426240.33513517763563955No Hit
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG323510.2543627564679658No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGCGTAGCGTGTAGATCT203130.15971285809198446Illumina Single End PCR Primer 1 (97% over 34bp)
CTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCT170850.13433240685775388No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA132390.10409287295228585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT243700.014.0343488
GAGCGTC261250.013.11912259
GATCGGA273000.012.6829761
AAGAGCG280450.012.4010317
TCGGAAG288500.011.979473
ATCGGAA326450.010.8302742
GAAGAGC332550.010.6095016
TAGCGTG68850.09.76740735-39
GGAAGAG370450.09.4655675
TAGATCT78900.09.106976540-44
CGGAAGA393550.08.7453454
TAGCGTA80100.08.66519230-34
AGTGTAG98500.07.22225525-29
AGCGTAG99300.07.120260230-34
AGATCTC103200.07.018286745-49
GTAGATC109400.06.804933540-44
GCGTGTA105300.06.75561635-39
GAGTGTA106700.06.640224525-29
GTGTAGA119800.06.40650340-44
GGTACTA32251.70985E-106.2759081
Produced by FastQC (version 0.11.5)